THE ALBUMIN AND GLOBULIN CONTENT OF HUMAN BLOOD SERUM
نویسندگان
چکیده
منابع مشابه
Serum Albumin and Globulin
Hundreds of proteins are dissolved in the plasma. By measuring the concentration of these proteins, the clinician can obtain information regarding disease states in different organ systems. The measurement of protein is done on serum, which is the fluid that remains after plasma has clotted, thus removing fibrinogen and most of the clotting factors. Total protein content provides some informati...
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THE SEPARATION of albumin from its associated globulins in serum or plasma has exercised physiologists and biochemists for many years. Several methods are available for assessing the albumin content of serum by prior precipitation of globulins (1-9). In 1949 Martin and Morris (10) examined a series of methods (1, 2, 5-7) and concluded, by comparison with the results of “free” electrophoresis, t...
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Thermal conformational changes in human serum albumin (HSA) in present with a 10 mM phosphate buffer, at pH=7 have been investigated via circular dichroism (CD) and UV spectroscopic methods. The results indicate that temperature in a range of 25oC to 55oC could induce a reversible conformational change in the structure of HSA. The HSA phase transition corresponds to the physiological and patho...
متن کاملThe Effects of Acetaminophen on Human Serum Albumin (HSA)
Thermal conformational changes in human serum albumin (HSA) in present with a 10 mM phosphate buffer, at pH=7 have been investigated via circular dichroism (CD) and UV spectroscopic methods. The results indicate that temperature in a range of 25oC to 55oC could induce a reversible conformational change in the structure of HSA. The HSA phase transition corresponds to the physiological and patho...
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Although the determination of the albumin and globulin contents of human serum by neutral salt fractionation, as in Howe’s method (I), has proved useful because of its simplicity, such methods have definite limitations. Butler (2) has shown that the albumin-globulin separation by neutral salt precipitation is not’ sharp and that these fractions overlap one another grossly. Leutscher (3) has als...
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ژورنال
عنوان ژورنال: Archives of Internal Medicine
سال: 1916
ISSN: 0730-188X
DOI: 10.1001/archinte.1916.00080170028003